12282s  (Cell Signaling Technology Inc)

Journal: Exploration

Article Title: Soluble Nanomedicine Against Soluble Neurosensitizers to Effectively Relieve Osteoarthritis Pain and Joint Damage

doi: 10.1002/exp2.70233

Figure Lengend Snippet: Therapeutic effect of LAC in the monoiodoacetate mouse model. (A) H&E staining of the joint from MIA mice after different treatments. (B) Quantitative analysis of the synovitis score. (C) Representative images of SO&FG‐stained knee joint sections from MIA mice with different treatments. (D) The severity of OA‐like lesions was analyzed using the OARSI scoring system. (E) Immunofluorescence imaging of iNOS (red)/CD206 (green) within the knee joints of mice with different treatments. (F) Corresponding quantification of the MFI of CD206 in the synovium of mice with different treatments. (G) Immunohistochemical staining of COX‐2, TNF‐α, and TRAP in the knee joints of mice with different treatments. (H,I) Quantification of the relative density of COX‐2 + (H) and TNF‐α + (I) cells in the knee joints of mice with different treatments. n = 5 in B and D, and n = 3 in F, H, and I, mean ± SD. The significant differences are determined by one‐way ANOVA analysis with Tukey's multiple comparison test. ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns stands for no significant difference.

Article Snippet: Proteins in equivalent amounts were then separated on a 10% sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred to the PVDF membrane, followed by blocking in TBS‐T solution containing 5% skim milk for 1 h. Subsequently, the membrane was incubated with primary antibody against COX‐2 (66351‐1‐Ig, Proteintech), iNOS (80517‐1‐RR, Proteintech), and CD206 (60143‐1‐Ig, Proteintech) overnight at 4°C.

Techniques: Staining, Analysis, Immunofluorescence, Imaging, Immunohistochemical staining, Comparison

Journal: Frontiers in Immunology

Article Title: Inflammatory landscape of Gardnerella vaginalis -associated bacterial vaginosis in rats: an integrated multi-omics analysis

doi: 10.3389/fimmu.2026.1914434

Figure Lengend Snippet: Upregulation of proteins associated with uterine TLR2/4–NF-κB–COX-2 inflammatory signaling in rats with Gardnerella vaginalis -associated bacterial vaginosis. (A) Relative protein abundance of Toll-like receptor 2 (TLR2), TLR4, phospho-NF-κB p65 at serine 536 (pS536-p65) and cyclooxygenase-2 (COX-2) in uterine tissues. Data are presented as the means ( X ¯ ) ± standard error of the mean (SEM); n = 3 independent uterine tissue samples per group. Statistical significance was determined using Student’s t-tests . Complete densitometric values are provided in Supplementary Table 11 . (B) Representative Western blot images. Protein levels were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Original uncropped Western blot images are provided in Supplementary Figure 1 .

Article Snippet: After blocking with a rapid blocking buffer (Epizyme, Shanghai, China), the blots were incubated overnight at 4 °C with the following primary antibodies: TLR2 (Abcam, Cambridge, UK; dilution 1:1000), TLR4 (Proteintech, Hubei, China; dilution 1:1000), phospho-NF-κB p65 at serine 536 (pS536-p65; Proteintech; dilution 1:2000), cyclooxygenase-2 (COX-2; Proteintech; dilution 1:1000), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Proteintech; dilution 1:10000).

Techniques: Quantitative Proteomics, Tissue, Western Blot